Axis BTA 2100D Instrukcja Operatora Pobierz pdf (Strona 215)

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Under the Isolation header, enter the Isolation Window (m/z).

The full mass isolation window range is 1.0 to 14.0 m/z; the actual range is mass

dependent. The default value is 3.0 m/z. Integral and fractional mass isolation

windows are both accepted. If mass isolation windows smaller than 1.5 m/z are

used, the exact mass of the parent ion should be entered in Parent Ion Mass.

NOTE: When first developing a method, you may wish to start with a mass

isolation window of 3 m/z to ensure that the ion is centered in the window, e.g.,

given a parent ion mass of 502 m/z and a mass isolation window of 3 m/z, ions of

masses 501, 502, and 503 m/z would be isolated. If there is a need to reduce the

window because of interfering ions, this should be done in a later step because

the customized parameters may require optimization.

Some ions are more easily dissociated during the isolation step. Therefore,

increasing the window will reduce the ion loss by dissociation at the expense of

selectivity.

Under the Dissociation header, select the Waveform Type by clicking on

nonresonant or resonant excitation.

The nonresonant method is usually used first because it is easier.

Excitation Storage Level (resonant or nonresonant excitation)

Definition: the rf storage level in m/z when the dissociation waveform is applied

following isolation.

The excitation storage level range depends on the parent mass, but the storage

level must lie more than 2 mass units below the lowest product ion value. The

default value is 48 m/z for both resonant or nonresonant modes.

Use the "q" Calculator for suggested excitation storage levels.

NOTE: Set the excitation storage level to avoid ejection of the lowest mass

product ion. If, for example, a parent ion of 403 m/z dissociated to product ions

with m/z values of 350, 200, and 131, you would not want to use a storage level

value above 126 m/z.

Excitation Amplitude in volts.

The amplitude range for nonresonant excitation is 0 to 100 volts. For resonant

excitation, the range is 0 to 60 volts. The default values are 20 volts for the

nonresonant excitation method and 0.2 volts for the resonant excitation method.

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