Axis BTA 2100D Instrukcja Operatora Pobierz pdf (Strona 437)

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GC Data Handling Fundamentals

Introduction

Even after optimizing your chromatographic conditions to provide the best

separation possible, you may still have unresolved peaks or other situations

requiring special post-run Data Handling operations. For instance, you may want

to exclude solvent peaks from the total area when doing area percent

calculations. You may want to ensure that a very small peak in one part of the

chromatogram is detected, and not rejected as a noise peak. Another common

use for special post-run Data Handling is to fine tune your quantitation method.

This section will provide an overview of both how the Varian MS Workstation

software performs the peak processing and how you can use the software to

most efficiently meet your Data Handling needs.

Refer to the Calibration section for more information on calibration and

verification procedures. Refer to the Regulatory Compliance Manual for a

complete description of Data Handling algorithms and calculations. Refer to the

on-line help in Interactive Graphics for descriptions of all of the fields in the Data

Handling section of the Method.

Performing a Pilot Run

You may wish to use a pilot run to adjust the Data Handling parameters to their

proper settings. You can use Interactive Graphics to edit the Data Handling

section of your Method, and save this along with the Instrument Control sections

for automatic data analysis on your production runs.

Data Handling Methods can be developed using an iterative approach. You

change Method parameters, then reintegrate, and then determine if the results of

your changes are appropriate. If not, you repeat these steps until you have the

desired results. The Interactive Graphics application in the Varian MS

Workstation is designed to facilitate this iterative process. You can view your pilot

run and create a Peak Table by clicking on peaks of interest with the mouse. You

can add timed integration events graphically using the Interactive Time Events

window. You can even generate and review calibration curves used to calculate

results. Refer to the section Interactive Graphics for more information on these

procedures.

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