Axis BTA 2100D Instrukcja Operatora Pobierz pdf (Strona 219)

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Dissociation

Excitation Time: The excitation time is the time required for collision-induced

dissociation (CID) by ion excitation. The excitation time range is 0 to 1000 msec.

The default excitation time is 20 msec for both resonant and nonresonant

excitation.

Customize Resonant Method

NOTE: Be sure that you have specified the parent ion mass before opening the

Customize Parameters window.

Customize Parameters window is separated into three sections. Ionization,

Isolation and Dissociation.

Ionization

Ionization Storage Level: The m/z value at which the rf is maintained during

ionization and the coarse isolation step. The range is 35 to 160 m/z, with a

default value of 48 m/z.

Ejection Amplitude: the amplitude (in volts) of the ejection waveform during the

coarse isolation step. The default value will work for most ions. For certain ions

that are less stable, this value may need to be reduced in order to minimize the

amount of energy imparted to the parent ion during isolation. The range of the

ejection amplitude is from 0 to 60 volts, with a default value of 20 volts.

Isolation

Low Edge Offset: This parameter provides a fine adjustment for the isolation

window to optimize the ejection of the mass just below the parent ion mass. The

low edge offset range is 0 to 20 steps. The default value is 6.

Large positive values widen the isolation window towards low masses.

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