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Auto Tune Checks
In the top center of the screen you will see 4 check boxes. These are the auto
tune steps that are to be performed. If your system does not contain SIS, or
MS/MS the Trap Function Calibration does not need to be performed. To perform
the auto tune click the check boxes and click Start Auto Tune.
Each one of the items that you selected in the check boxes will now be executed.
If you checked the single step button, a single step will be executed but will not
proceed to the next until the continue button is clicked. You can stop the auto
tune at any time by clicking on the Reset button.
Air/Water Check
When you select Air/Water Check, you obtain a screen from which you can
determine the relative amount of air and water vapor in the vacuum manifold.
When you display the Air/Water screen, the following happens automatically: The
filament, electron multiplier, and rf voltage are turned on; AGC is turned off; and
the scan range is set to display the air/water spectrum (14 to 44 m/z). To
determine the air background, the width of the peak for mass 28 at 10% peak
height is calculated. To determine the water background, the ratio of mass 19 to
mass 18 is calculated.
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